02137naa a2200229 a 450000100080000000500110000800800410001902200140006010000200007424501200009426000090021430000230022350000480024652014210029465000230171565300180173865300240175670000130178070000220179370000180181577300740183310125042019-10-30       2004    bl uuuu     u00u1 u    #d  a0394-92571 aDALLA RIZZA, M.  aPlant reproductive system determination under field conditions based on condominant markers.h[electronic resource]  c2004  c2-s2.0-33748791330  aArticle history: Received February 9, 2004.  aABSTRACT. Two co-dominant PCR-based molecular tools, sequence characterized amplified regions (SCAR) markers and restriction enzymes mediated markers, sequence characterized amplified regions (CAPS) markers, have been utilized to study the mating system in Lotononis bainesii Baker. Randomly amplified polymorphic DNA (RAPD) phenotypes - bands on a gel - were selected to transform into specific SCAR genotypes - allelic constitution at a set of loci. Moreover, some monomorphic SCAR genotypes, were made polymorphic through the use of restriction enzymes, in order to analyze allelic contribution on progenies. Based on stringent annealing temperatures that generate only specific DNA fragments and only in those individuals possessing the appropriate allele, it was possible to recognize alleles from different donors. This approach overcomes the limitation of different dominant markers and has the advantage of analyzing a single genetic locus PCR-based, avoiding the necessity of complex and time consuming controlled crosses. Thus, more suitable for reproductive system studies based on the use of molecular characterized plants that acts as "tester genotypes". In this article we discuss the advantages of using two independent and conclusive molecular approaches based on SCAR and CAPS markers to determine plant breeding systems. We also show that allogamy is the mode of reproduction of Lotononis bainesii.  aLOTONONIS BAINESII  aMating system  aSCAR/CAPS genotypes1 aREAL, D.1 aQUESENBERRY, K.H.1 aALBERTINI, E.  tJournal of Genetics and Breeding, 2004, Volume 58, Issue 1, pp 47-54.