02264naa a2200289 a 450000100080000000500110000800800410001902200140006002400350007410000250010924501120013426000090024650002760025552011670053165000180169865000220171665000320173865000130177065000230178370000140180670000240182070000150184470000170185970000170187670000230189377300580191610507032019-10-09       2012    bl uuuu     u00u1 u    #d  a0304-86087 a10.1007/s00705-012-1262-62DOI1 aMÁRQUEZ-MARTÍN, B.  aDiverse population of a new bipartite begomovirus infecting tomato crops in Uruguay.h[electronic resource]  c2012  aArticle history: Received: 5 October 2011 / Accepted: 17 January 2012 / Published online: 1 March 2012. The nucleotide sequences reported in this work are available in the GenBank database under the accession numbers JN381813 to JN381829. Contains supplementary material.  aABSTRACT.  Several isolates of a novel begomovirus were characterized from tomato samples collected in northern Uruguay exhibiting disease symptoms associated with Bemisia tabaci infestations. Analysis of full-length sequences of DNA-A and DNA-B components revealed the presence of a new begomovirus with the typical genome organization of a New World begomovirus, for which the name tomato rugose yellow leaf curl virus (ToRYLCV) is proposed. A high degree of nucleotide sequence diversity was found for both components, suggesting the presence of a diverse virus population. Recombination analysis suggested relationships of ToRYLCV to begomoviruses reported from the New World. Although common regions from DNA-As and DNA-Bs were surprisingly divergent for a cognate pair, a DNA-A and DNA-B pair cloned from one sample were infectious in Nicotiana benthamiana and tomato and reproduced symptoms observed in field-infected tomato plants, suggesting that ToRYLCV is the causal agent of the disease observed. This is the first report of a begomovirus infecting tomato crops in Uruguay and of the presence of begomovirus in this country.  © 2012 Springer-Verlag.  aBIODIVERSIDAD  aCULTIVO DE TOMATE  aENFERMEDADES DE LAS PLANTAS  aGENETICA  aGENETICA MOLECULAR1 aMAESO, D.1 aMARTÍNEZ-AYALA, A.1 aBERNAL, R.1 aFEDERICI, M.1 aVINCELLI, P.1 aNAVAS-CASTILLO, J.  tArchives of Virology, 2012gv.157, no.6, p.1137-1142.