02189naa a2200301 a 450000100080000000500110000800800410001902400250006010000170008524500970010226000090019950001060020852012780031465000130159265000110160565000110161665000170162765300200164465300210166465300160168565300230170165300170172470000190174170000190176070000190177970000170179877300720181510538702018-10-09       2012    bl uuuu     u00u1 u    #d7 a10.1071/RD112812DOI1 aVIÑOLES, C.  aEmbryo losses in sheep during short-term nutritional supplementation.h[electronic resource]  c2012  aHistory article: Received 4 November 2011; accepted 15 February 2012; published online 30 March 2012.  aTo test whether a nutritional supplement fed from 6 days before until 15 days after insemination reduces progesterone concentrations and increases embryo losses, Merino ewes were artificially inseminated (Day 0). Control ewes (n¼116) were not supplemented whereas Lupin6 ewes (n¼112) were supplemented with 500 g lupin grain daily for 6 days before insemination, and Lupin6þ15 ewes (n¼122) from 6 days before until 15 days after insemination. There were no major differences between treatment groups in progesterone concentrations over the first 17 days of pregnancy. Embryo losses over Days 10?17 were lower in the Lupin6þ15 than in the Control and Lupin6 groups, but the opposite occurred from Day 17?30. The concentrations of insulin and IGF-I were higher in Lupin6þ15 ewes on Days 5, 12 and 17, compared with Lupin6 and Control ewes, while leptin concentrations decreased by Day 17 in the Lupin6þ15 group. We conclude that feeding ewes for 15 days after mating improved embryo survival, which was associated with an increase in the concentrations of metabolic hormones and lower progesterone concentrations. However, the decrease in leptin concentrations promoted by the interruption of supplementation seems be linked to increased embryo mortality up to Day 30.  aHORMONAS  aMERINO  aOVINOS  aPROGESTERONA  aEMBRYO SURVIVAL  aLUPIN SUPPLEMENT  aMERINO EWES  aMETABOLIC HORMONES  aPROGESTERONE1 aGLOVER, K.M.M.1 aPAGANONI, B.L.1 aMILTON, J.T.B.1 aMARTIN, G.B.  tReproduction, Fertility and Development, 2012gv. 24, p. 1040-1047.