02650naa a2200373 a 450000100080000000500110000800800410001902400270006010000160008724501020010326000090020550002500021452013960046465000280186065000330188865300100192165300180193165300280194965300140197765300080199165300340199965300150203365300120204865300100206070000150207070000180208570000180210370000190212170000200214070000170216070000180217770000160219577300650221110582262019-10-09       2018    bl uuuu     u00u1 u    #d7 a10.1111/jam.136912DOI1 aCASTELL, N.  aPrevalence and viability of group A rotavirus in dairy farm water sources.h[electronic resource]  c2018  aHistory article: Issue online: 15 February 2018; Version of record online: 7 February 2018;Accepted manuscript online: 9 January 2018 Manuscript Accepted: 2 January 2018;Manuscript Revised: 30 December 2017;Manuscript Received: 17 November 2017.  aAbstract. Aim: To analyze group A rotavirus (RVA) environmental contamination in waters used for  calves? consumption and to assess viral viability in dairy farm water sources.  Methods and Results: We analyzed 202 samples of water used for calves? consumption and  RVA was detected by RT-qPCR in 35.1% (95%CI: 28.9?42.0%). A marked pattern of seasonality  was observed with higher frequency of detection in colder than warmer months (p=0.002).  There was no association between viral load and season or between the number of milking  cows in the herd and the detection of RVA in the farm. The viability of the RVA particles  detected was confirmed by isolation of RVA in cell culture from 5/10 water samples.  Furthermore, an RVA waterborne outbreak of neonatal calf diarrhea was described.  Conclusions: We demonstrate that RVA is frequent in dairy farm waters, and that the virus is  infectious and capable of generating a diarrhea outbreak.  Significance and Impact of Study: Neonatal diarrhea syndrome leads to economic losses to the  livestock industry worldwide. To determine transmission routes is essential to take action in  this regard and reduce the impact that this syndrome has for the livestock production. The  results obtained in this work alerts the dairy industry and highlights that mitigation strategies  are crucial to improve the microbiological quality of this water.  aCONTAMINACION AMBIENTAL  aENFERMEDADES DE LOS ANIMALES  aDAIRY  aENVIRONMENTAL  aPLATAFORMA SALUD ANIMAL  aROTAVIRUS  aRVA  aSÍNDROME DE DIARREA NEONATAL  aVETERINARY  aVIRUSES  aWATER1 aSCHILD, C.1 aCAFFARENA, D.1 aGIANNITTI, F.1 aARMENDANOE, J.1 aRIET-CORREA, F.1 aVICTORIA, M.1 aPARREÑOC, V.1 aCOLINAA, R.  tJournal of Applied Microbiology, 124gn.3, p. 922-929, 2018.