02685naa a2200385 a 450000100080000000500110000800800410001902200340006002400350009410000190012924500880014826000090023650000490024552015860029465000290188065000100190965000160191965300190193565300200195465300080197465300360198265300310201865300280204965300170207765300180209465300180211270000140213070000180214470000220216270000160218470000150220070000200221570000180223577300460225310588332019-10-09       2018    bl uuuu     u00u1 u    #d  a0301-486X / Online: 1573-08327 a10.1007/s11046-018-0274-52DOI1 aSILVEIRA, C.S.  aA case of prototheca zopfii genotype 1 infection in a dog (Canis lupus familiaris).  c2018  aArticle history: First Online: 06 June 2018.  aABSTRACT.  Protothecosis is a rare disease caused by environmental algae of the genus Prototheca. These are saprophytic, non-photosynthetic, aerobic, colorless algae that belong to the Chlorellaceae family. Seven different species have been described. Prototheca zopfii genotype 2 and P. wickerhamii are most commonly involved in pathogenic infections in humans and animals. The objective of this work is to describe, for the first time, a case of protothecosis caused by P. zopfii genotype 1 in a dog. The dog, a 4-year-old mix bred male, was presented to a veterinary clinic in Montevideo, Uruguay, with multiple skin nodules, one of which was excised by surgical biopsy. The sample was examined histologically and processed by PCR, DNA sequencing, and restriction fragments length polymorphisms for the detection and genotyping of P. zopfii. In addition, transmission electron microscopy and scanning electron microscopy were performed. Histology showed severe ulcerative granulomatous dermatitis and panniculitis with myriads of pleomorphic algae. Algal cells were 4?17 µm in size, with an amphophilic, 2?4-µm-thick wall frequently surrounded by a clear halo, contained flocculant material and a deeply basophilic nucleus, and internal septae with daughter cells (endospores) consistent with endosporulation. Ultrastructurally, algal cells/endospores at different stages of development were found within parasitophorous vacuoles in macrophages. Prototheca zopfii genotype 1 was identified by molecular testing, confirming the etiologic diagnosis of protothecosis. © Springer  aENFERMEDADES INFECCIOSAS  aPERRO  aSUD AMERICA  aAlgal diseases  aAlgal infection  aDog  aENFERMEDADES CAUSADAS POR ALGAS  aGenotypic characterization  aPLATAFORMA SALUD ANIMAL  aPROTOTECOSIS  aProtothecosis  aSOUTH AMERICA1 aCESAR, D.1 aKEATING, M.K.1 aDELEON-CARNES, M.1 aARMIÉN, A.1 aLUHERS, M.1 aRIET-CORREA, F.1 aGIANNITTI, F.  tMycopathologia, June 2018. [First Online]