02929naa a2200361 a 450000100080000000500110000800800410001902000220006002200480008202400420013010000300017224501050020226000090030749000320031650003420034852015250069065300160221565300190223165300150225065300190226565300200228465300080230465300100231265300100232270000270233270000220235970000260238170000280240770000190243570000230245470000230247777300670250010600122019-08-16       2019    bl uuuu     u00u1 u    #d  a978-94-62612-36-5  a0567-7572 (print) // 2406-6168 (electronic)7 a10.17660/ActaHortic.2019.1239.252DOI1 aHERNÁNDEZ-RODRÍGUEZ, L.  aThe viral complex associated with mealybug wilt disease of pineapple in Cuba.h[electronic resource]  c2019  a(Acta Horticulturae; 1239).  aArticle history: Published 30 april 2019. In: Acta Horticulturae (ISHS) 1239: IX International Pineapple Symposium. Editors: D.P. Bartholomew, D.H. Reinhardt, F.V. Duarte Souza. Convener: J. Valdés-Infante Herrero . Funding text: Part of these results are included in the Ph.D. thesis document of Lester Hernández-Rodrı́guez,  aABSTRACT. Mealybug wilt of pineapple (MWP) disease has been associated with the infection by a complex of ampeloviruses (Pineapple mealybug wilt-associated virus 1 (PMWaV-1), PMWaV-2, PMWaV-3, PMWaV-4 and PMWaV-5)) (Closteroviridae) and badnaviruses (Pineapple bacilliform CO virus (PBCOV) and Pineapple bacilliform ER virus (PBERV) (Caulimoviridae)). In Cuba, PMWaV-1, PMWaV-2, PMWaV-3 and PBCOV are widespread in commercial pineapple fields, but the association of these viruses with the aetiology of the disease remains unknown. During 2009 to 2012, a survey to detect PMWaV-1, PMWaV-2 and PMWaV-3 by RT-PCR, and PBCOV by non-radioactive Dot Blot hybridization was carried out on samples collected from asymptomatic or MWP symptomatic pineapple plants from 24 commercial fields in 10 provinces and Isla de la Juventud. PMWaV-1, PMWaV-2, PMWaV-3, and PBCOV occurred in simple or mixed infections, whereas PMWaV-2 was the viral species most frequently detected in symptomatic plants. The positive or negative diagnostic results of the RT-PCR to detect PMWaV-2 was more reliably associated with the MWP symptomatic or asymptomatic status of the sampled plants, respectively, than those from PMWaV-1, PMWaV-3 or PBCOV detection techniques. This work discloses the correlation between the infection by PMWaV-2 and the presence of MWP symptoms in Cuba, supporting previous studies indicating the major role of this virus triggering MWP disease.  © 2019 International Society for Horticultural Science. All rights reserved.  aAMPELOVIRUS  aANANAS COMOSUS  aBADNAVIRUS  aCAULIMOVIRIDAE  aCLOSTEROVIRIDAE  aMWP  aPBCOV  aPMWaV1 aRAMOS-GONZÁLEZ, P. L.1 aSISTACHS-VEGA, V.1 aZAMORA-RODRÍGUEZ, V.1 aBATISTA-LE RIVEREND, L.1 aRAMOS-LEAL, M.1 aPEÑA-BÁRZAGA, I.1 aLLANES-ALVAREZ, Y.  tActa Horticulturae, 30 April 2019, Volume 1239, pages 203-212.