02463naa a2200289 a 450000100080000000500110000800800410001902400320006010000200009224501370011226000090024930000100025850001080026852014720037665300170184865300230186565300280188865300090191665300190192565300350194465300190197970000150199870000160201370000150202970000150204477301140205910615802020-12-16       2020    bl uuuu     u00u1 u    #d7 a10.1093/femsec/fiaa2072DOI1 aGARAYCOCHEA, S.  aSoil structure, nutrient status and water holding capacity shape uruguayan grassland prokaryotic communities.h[electronic resource]  c2020  a32 p.  aArticle history: Received: 13 February 2020 // Accepted: 08 October 2020 // Published: 10 October 2020.  aSoil microbial communities play critical roles in maintaining natural ecosystems such as the Campos biome grasslands of southern South America. These grasslands are characterized by a high diversity of soils, low available phosphorus (P) and limited water holding capacity. This work aimed to describe prokaryotic communities associated with different soil types and to examine the relationship among these soil communities, the parent material and the soil nutrient status. Five Uruguayan soils with different parent material and nutrient status, under natural grasslands, were compared. The structure and diversity of prokaryotic communities were characterized by sequencing 16S rRNA gene amplicons. Proteobacteria, Actinobacteria, Firmicutes,Verrucomicrobia, Acidobacteria, Planctomycetes and Chloroflexi were the predominant phyla. Ordination based on several distance measures was able to discriminate clearly between communities associated with different soil types. Edge-PCA phylogeny-sensitive ordination and differential relative abundance analyses identified Archaea and the bacterial phyla Firmicutes, Acidobacteria, Actinobacteria and Verrucomicrobia as those with significant differences among soil types. Canonical analysis of principal coordinates identified porosity, clay content, available P, soil organic carbon and water holding capacity as the main variables contributing to determine the characteristic prokaryotic communities of each soil type.  aCAMPOS BIOME  aNATURAL GRASSLANDS  aPROKARYOTIC COMMUNITIES  aSOIL  aSOIL NUTRIENTS  aSOIL PHYSICOCHEMICAL VARIABLES  aSOIL STRUCTURE1 aROMERO, H.1 aBEYHAUT, E.1 aANDREW, L.1 aALTIER, N.  tFEMS Microbiology Ecology, December 2020gv. 96, no. 12, fiaa207. DOI: https://doi.org/10.1093/femsec/fiaa207