02535naa a2200265 a 450000100080000000500110000800800410001902400390006010000170009924501910011626000090030750002500031652013870056665000190195365000160197265300160198865300150200465300250201965300220204470000160206670000160208270000160209870000210211477301340213510621202021-08-10       2021    bl uuuu     u00u1 u    #d7 a10.1080/01647954.2021.19141582DOI1 aMITTON, G.A.  aL925V mutation in voltage-gated sodium channel of Varroa destructor populations from Argentina and Uruguay, with different degree of susceptibility to pyrethroids.h[electronic resource]  c2021  aArticle history: Accepted 29 March 2021/Published online 13 May 2021.  This work was supported by the Agencia Nacional de Promoción Científica y Tecnológica under Grant [PICT-2015-1807]. GA Mitton is a doctoral fellow from CONICET, Argentina.  aABSTRACT: This study aimed to detect mutations associated with pyrethroid resistance mechanism in different populations of Varroa destructor from Uruguay and Argentina with different degree of susceptibility to this family of acaricides. Bioassays were performed to evaluate the degree of susceptibility to flumethrin  and tau-fuvalinate; then mites were studied in order to determine the presence or absence of the resistance-associated mutations by sequencing a region of the sodium channel gene. In the mites studied from Colonia, which showed resistance to flumethrin and susceptibility to tau-fluvalinate in the bioassays, L925V mutation was observed in heterozygous and homozygous state. The presence of the wild-type allele in homozygous state was identified in all mites from Treinta y Tres, in which acaricides have never been used. The mite population from Mar del Plata through bioassays were determined as susceptible to both pyrethroids, however, genotyping results showed the presence of heterozygous individuals (925 V/L) as well as homozygous wild type (925L), while homozygous resistant  individuals (925V) were present at low frequency. This would suggest that this population presents a great potential to give a generalized resistance to pyrethroids. This study represents the first report of a L925V mutation in resistant mites to flumethrin from South America.  aAPIS MELLIFERA  aRESISTENCIA  aPyrethroids  aRESISTANCE  aSynthetic acaricides  aVARROA DESTRUCTOR1 aQUINTANA, S1 aMENDOZA, Y.1 aEGUARAS, M.1 aRUFFINENGO, S.R.  tInternational Journal of Acarology,  Volume 47, Issue 5,  Pages 374-380, 2021. Doi: https://doi.org/10.1080/01647954.2021.1914158