02910naa a2200325 a 450000100080000000500110000800800410001902200140006002400380007410000190011224501070013126000090023850005320024752014250077965300080220465300080221265300180222065300210223865300230225965300160228270000190229870000210231770000190233870000170235770000280237470000170240270000170241970000140243677301340245010622162021-07-02       2021    bl uuuu     u00u1 u    #d  a1877-959X7 a10.1016/j.ttbdis.2021.1017652DOI1 aDE LORENZO, C.  aRangelia vitalii in free-living crab-eating foxes (Cerdocyon thous) in Uruguay.h[electronic resource]  c2021  aArticle history: Received 25 November 2020; Received in revised form 1 May 2021; Accepted 13 May 2021; Available online 4 June 2021. This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico ( CNPq ) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ( CAPES 001 ). Corresponding author: Soares, J.F.; Laboratório de Protozoologia e Rickettsioses Vetoriais (ProtozooVet), UFRGS, Avenida Bento Gonçalves, Porto Alegre, Rio Grande do Sul, Brazil; email:joao.soares@ufrgs.br  aABSTRACT - Rangelia vitalii is a protozoan parasite that causes a hemorrhagic and hemolytic disease in dogs known as rangeliosis. Current reports of the disease are concentrated in the southern and southeastern regions of Brazil, as well as in Uruguay, Argentina, and Paraguay, and mainly concern domestic dogs. South American wild canids, such as the crab-eating fox (Cerdocyon thous), the pampas fox (Lycalopex gymnocercus), and the maned wolf (Chrysocyon brachyurus) may also be affected, although existing reports are restricted to Brazil. The present study aimed to detect R. vitalii parasitism in the Uruguayan wild fox population. DNA extracted from the blood and/or spleen samples of road-killed C. thous and L. gymnocercus found in northern Uruguay were subjected to polymerase chain reaction (PCR) to amplify a 551-bp fragment of the Rangelia 18S rRNA gene. A total of 62 wild canids, including 38 C. thous and 24L. gymnocercus, were analyzed. Five crab-eating fox samples (13.2%) were positive for R. vitalii, with 99.5?100% identity between the sequences. All samples from pampas fox tested negative for R. vitalii. When compared with the R. vitalii sequences available in GenBank, a similarity of 98.9?100% was revealed. Molecular analysis results suggest that R. vitalii is circulating in the crab-eating fox population in Uruguay; however, its veterinary relevance for these foxes remains unknown. © 2021  aDNA  aDog  aHemoparasites  aRangelia vitalii  aTick-borne disease  aWild canids1 aBOABAID, F. M.1 aOLIVEIRA, L.G.S.1 aBIANCHI, M. V.1 aFÉLIX, M.L.1 aARMÚA-FERNÁNDEZ, M.T.1 aSOARES, J.F.1 aVENZAL, J.M.1 aSONNE, L.  tTicks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101765. Doi: https://doi.org/10.1016/j.ttbdis.2021.101765