02795naa a2200349 a 450000100080000000500110000800800410001902400360006010000160009624502100011226000090032250002500033152014060058165300180198765300160200565300230202165300490204465300310209365300180212465300180214270000160216070000160217670000140219270000180220670000180222470000180224270000160226070000200227670000160229670000180231277301150233010622842022-06-27       2021    bl uuuu     u00u1 u    #d7 a10.1007/s11033-021-06492-w2DOI1 aMANISSE, N.  aMolecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probesba contribution to their molecular epidemiology.h[electronic resource]  c2021  aArticle history: Received: 24 November 2020 / Accepted: 11 June 2021.  This study obtained fnancial support from Programa de  Desarrollo de Ciencias Básicas (PEDECIBA) and Agencia Nacional de Investigación e Innovación (FSSA_X_2014_1_106026).  aAbstract: Neospora caninum, Toxoplasma gondii and Hammondia spp. are coccidian parasites similar in morphology. Molecular techniques are necessary to detect parasite DNA isolated from stool samples in wild canids because they were reported as defnitive hosts of N. caninum life cycle. The objective of this study was to develop a highly sensitive and accurate molecular method for the identifcation of coccidian Apicomplexa parasites in crab-eating fox (Cerdocyon thous) and pampas fox (Lycalopex gymnocercus). Tissue samples from road-killed animals (pampas fox=46, crab-eating fox=55) and feces (pampas fox=84, crab-eating fox=2) were collected, and species were diagnosed through molecular assay. PCR was used for the amplifcation of a fragment of the coccidian Apicomplexa nss-rRNA gene. Additionally, we developed a novel realtime PCR TaqMan? probe approach to detect T. gondii- Hammondia spp. and N. caninum. This is the frst report of N. caninum DNA in pampas fox feces (n=1), thus it was also detected from pampas fox tissues (n=1). Meanwhile, T. gondiiwas found in tissues of pampas (n=1) and crab-eating (n=1) foxes and H. trifttae in one crab-eating fox tissue. Despite the low percentage (2.5%) of positive samples, the molecular method developed in this study proved to be highly sensitive and accurate allowing to conduct an extensive monitoring analysis for these parasites in wildlife.  aHammondia spp  aNEOSPOROSIS  aNeotropical canids  aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL  aPLATAFORMA DE SALUD ANIMAL  aReal-time PCR  aTOXOPLASMOSIS1 aCABRERA, A.1 aHERNÁN, J.1 aCOSSE, M.1 aGIANNITTI, F.1 aFRANCIA, M.E.1 aGONZÁLEZ, T.1 aIRIARTE, A.1 aRIET-CORREA, F.1 aROBELLO, C.1 aGONZÁLEZ, S.  tMolecular Biology Reports, Volume 48, Pages 5013–5021, 2021. Doi: https://doi.org/10.1007/s11033-021-06492-w