02485naa a2200241 a 450000100080000000500110000800800410001902400360006010000250009624501320012126000090025350000710026252016110033365300110194465300170195565300150197265300490198765300310203665300150206770000140208270000150209677301320211110623842022-11-29       2022    bl uuuu     u00u1 u    #d7 a10.1007/s12602-021-09834-z2DOI1 aFERNÁNDEZ-CIGANDA S  aProbiotic lactobacilli administration induces changes in the fecal microbiota of preweaned dairy calves.h[electronic resource]  c2022  aArticle history: Accepted 04 August 2021/ Published 14 August 2021  aAbstract: Early microbial colonization is a determinant factor in animal health, and probiotic administration has been demonstrated to modulate intestinal microbiota and promote health in dairy calves. The objective of this study was to evaluate changes in calves' fecal microbiota after the administration of two probiotic lactobacilli strains that had previously exhibited benefcial efects in calves' health in relation to neonatal calf diarrhea. An in vivo assay was performed with 30 newborn male Holstein calves that were divided into three groups. Two groups were orally administered with two diferent lactobacilli strains (Lactobacillus johnsonii TP1.6 or Limosilactobacillus reuteri TP1.3B), and the third was the control group. Calves (5 to 9 days old) were administered with freeze-dried bacteria once a day for 10 consecutive days. Feces samples were taken before the frst administration (day 0) and then again on days 10 and 21, and the V4 region of the bacterial 16S ribosomal gene was sequenced with an Illumina MiSeq 250 paired-end platform. The administration of both strains signifcantly afected the total  bacterial community composition, and the efect lasted for 11 days after the last dose. In particular, amplicon sequence variants related to Bifdobacterium and Akkermansia genera were signifcantly higher in both treated groups. Therefore, modulation of the intestinal microbiota is a potential mechanism of action behind the benefcial efects of these probiotic strains. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.  aCalves  aLactobacilli  aMicrobiota  aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL  aPLATAFORMA DE SALUD ANIMAL  aProbiotics1 aFRAGA, M.1 aZUNINO, P.  tProbiotics and Antimicrobial Proteins, 2022, volume 14, issue 5, pages 804-815. Doi: https://doi.org/10.1007/s12602-021-09834-z