02796naa a2200325 a 450000100080000000500110000800800410001902200140006002400270007410000180010124501630011926000090028250003300029152014360062165000100205765300240206765300090209165300410210065300140214165300250215565300240218065300190220470000300222370000170225370000190227070000210228970000200231070000140233077301260234410631542022-11-30       2022    bl uuuu     u00u1 u    #d  a0931-26687 a10.1111/jbg.126822DOI1 aBERTON, M. P.  aGenomic integration to identify molecular biomarkers associated with indicator traits of gastrointestinal nematode resistance in sheep.h[electronic resource]  c2022  aArticle history: Received 14 June 2021; Accepted 21 April 2022; First published 09 May 2022; Publisched online September 2022. -- Corresponding author:  Berton, M.P.; Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, Brazil; email:mapberton@gmail.com --  aABSTRACT.- This study aimed to integrate GWAS and structural variants to propose possible molecular biomarkers related to gastrointestinal nematode resistance traits in Santa Inês sheep. The phenotypic records FAMACHA, haematocrit, white blood cell count, red blood cell count, haemoglobin, platelets and egg counts per gram of faeces were collected from 700 naturally infected animals, belonging to four Brazilian flocks. A total of 576 animals were genotyped using the Ovine SNP12k BeadChip and were imputed using a reference population with Ovine SNP50 BeadChip. The GWAS approaches were based on SNPs, haplotypes, CNVs and ROH. The overlapping between the significant genomic regions detected from all approaches was investigated, and the results were integrated using a network analysis. Genes related to the immune system were found, such as ABCB1, IL6, WNT5A and IRF5. Genomic regions containing candidate genes and metabolic pathways involved in immune responses, inflammatory processes and immune cells affecting parasite resistance traits were identified. The genomic regions, biological processes and candidate genes uncovered could lead to biomarkers for selecting more resilient sheep and improving herd welfare and productivity. The results obtained are the start point to identify molecular biomarkers related to indicator traits of gastrointestinal nematode resistance in Santa Inês sheep. © 2022 Wiley-VCH GmbH.  aOVEJA  aCopy number variant  aGWAS  aGWAS - Genome-wide association study  aHaplotype  aIntegration analyses  aRun of homozygosity  aSystem biology1 aDA SILVA, ROSIANE PEREIRA1 aBANCHERO, G.1 aMOURÃO, G. B.1 aFERRAZ, J. B. S.1 aSCHENKEL, F. S.1 aBALDI, F.  tJournal of Animal Breeding and Genetics, 2022, Volume 139, Issue 5, pages 502-516. doi: https://doi.org/10.1111/jbg.12682