02862naa a2200373 a 450000100080000000500110000800800410001902200140006002400430007410000190011724501010013626000090023750002530024652014960049965300170199565300150201265300380202765300260206565300560209170000150214770000230216270000220218570000210220770000170222870000170224570000200226270000180228270000140230070000160231470000210233070000180235170000190236977301000238810651702025-04-28       2025    bl uuuu     u00u1 u    #d  a0001-706X7 a10.1016/j.actatropica.2025.1075952DOI1 aMORALES, D. F.  aFirst molecular detection of Babesia bigemina in horseflies from Uruguay.h[electronic resource]  c2025  aArticle history: Received 19 November 2024, Revised 17 February 2025, Accepted 21 March 2025, Available online 24 March 2025, Version of Record 29 March 2025. -- Corresponding author: E-mail address: diulimoralesfonseca@gmail.com (D.F. Morales). --  aABSTRACT.- Bovine babesiosis and anaplasmosis are primarily transmitted by the biological vector Rhipicephalus microplus. However, Anaplasma marginale may also be mechanically transmitted through the bites of haematophagous flies such as tabanids. While biological transmission by ticks is the predominant route for Babesia species, some reports suggest that mechanical transmission via fomites contaminated with merozoites may occur. Additionally, mechanical vectors such as tabanids may contribute to the spread of the disease. This study investigated the presence of Babesia bigemina and Babesia bovis in tabanids from three localities in Uruguay previously evaluated for the presence of A. marginale. We performed PCR amplification targeting the Rap-1a and gp45 genes, followed by Sanger sequencing. Our findings revealed that 4.21 % of the tabanids tested positive for B. bigemina, with the highest prevalence in Tacuarembó (28.57 %), followed by Paysandú (3.22 %) and Colonia (1.75 %). Dasybasis missionum from Tacuarembó, Poeciloderas lindneri from Paysandú, and Tabanus sp. from Colonia were positive. Additionally, a co-detection rate of 2 % with A. marginale was observed. These findings underscore the potential role of tabanids as vectors for Babesia bigemina, emphasizing the need for integrated vector control strategies in livestock management. © 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.  aAnaplasmosis  aBabesiosis  aMechanical transmission, tabanids  aPathogen co-detection  aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA1 aPARODI, P.1 aDOS REIS, L. F. V.1 aNASCIMENTO, M. C.1 aRODRIGUES, G. D.1 aNUNES, L. S.1 aMENCHACA, A.1 aRIET-CORREA, F.1 aKROLOW, T. K.1 aLUCAS, M.1 aSARAVIA, A.1 aDOMINGUES, W. B.1 aCAMPOS, V. F.1 aKRÜGER, R. F.  tActa Tropica, April 2025, Volume 264, 107595. https://doi.org/10.1016/j.actatropica.2025.107595