02169naa a2200241 a 450000100080000000500110000800800410001902200140006002400380007410000210011224501110013326000090024449000600025350004920031352008180080565300300162365300160165365300140166965300370168365300250172070000140174577301680175910651762025-05-02       2025    bl uuuu     u00u1 u    #d  a1064-37457 a10.1007/978-1-0716-4450-8_122DOI1 aARRUABARRENA, A.  aAgrobacterium-mediated transformation for gene editing tomato elite breeding lines.h[electronic resource]  c2025  aSpringer Protocols (Methods in Molecular Biology, 2911)  aChapter history: Published 28 March 2025. -- Correspondence: Arruabarrena, A.; Instituto Nacional de Investigación Agropecuaria, Estación Experimental INIA Salto Grande, Salto, Uruguay; email:aarruabarrena@inia.org.uy  -- Funding: The authors thank the INIA Tomato Breeding Program for providing elite genotypes for in vitro regeneration and transformation adjustments, and the INIA (L1 HO_20_0_00) and CSIC (CSIC I+D 1492, UdelaR) projects for funding. -- Publisher: Humana Press Inc.  aABSTRACT.- Genome editing success in crop species is largely dependent on the availability of highly efficient plant transformation protocols. Tomato (Solanum lycopersicum) was the first dicotyledonous crop to be successfully mutagenized using CRISPR-Cas9. Despite many efforts, no standardized, simple protocol is available for non-model tomato genotypes. With the increasing availability of gene editing tools, the transformation of elite tomato breeding lines has gained importance because mutant variants can be easily incorporated into breeding programs. This chapter describes a protocol for transforming and gene editing in elite tomato breeding lines, reaching 3.6% transformation efficiency. © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.  aAgrobacterium tumefaciens  aCRISPR-Cas9  aHypocotyl  aSISTEMA VEGETAL INTENSIVO - INIA  aSolanum lycopersicum1 aVIDAL, S.  tIn: Stange Klein, C. (eds). Agrobacterium. Methods in Molecular Biology, vol 2911. Humana, New York, NY. pp. 121-131.  https://doi.org/10.1007/978-1-0716-4450-8_12