03126naa a2200373 a 450000100080000000500110000800800410001902200140006002400530007410000140012724501440014126000090028550005610029452014310085565300230228665300150230965300130232465300560233765300280239365300250242170000140244670000160246070000170247670000170249370000290251070000180253970000140255770000130257170000160258470000180260070000160261870000150263477301030264910652982025-07-17       2025    bl uuuu     u00u1 u    #d  a0165-24787 ahttps://doi.org/10.1016/j.imlet.2025.1070522DOI1 aLORES, P.  aReactive oxygen species production by monocytes negatively correlates with disease activity in rheumatoid arthritis.h[electronic resource]  c2025  aArticle history: Received 21 January 2025, Revised 24 May 2025, Accepted 8 June 2025, Available online 8 June 2025, Version of Record 18 June 2025, Publication date December 2025. -- Corresponding author: T. Freire, Laboratorio de Inmunomodulación y Vacunas, Unidad Académica de Inmunobiología, Facultad de Medicina, Universidad de la República, Uruguay, Email: tfreire@fmed.edu.uy -- Funding: This work was supported by Comisión Sectorial de Investigación Científica (CSIC), PEDECIBA and Facultad de Medicina, UdelaR. -- Publisher: Elsevier B.V. --  aABSTRACT.- Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by severe joint inflammation, synovial hyperplasia and degradation of the cartilage and bone in the joint. Patients with RA have an amplified T helper (Th) 1 and Th17 immune response and production of autoantibodies by autoreactive B cells. In the joint, macrophages mediate bone destruction and maintain the inflammatory process in RA. There is an increasing body of evidence indicating that NADPH oxidase (NOX2)-rived reactive oxygen species (ROS), mainly produced by macrophages and neutrophils, may have effector functions in RA. In this work we characterized ROS production in both monocytes and macrophages in RA. Our results indicate that NOX2-dependent production of ROS attenuates inflammation and clinical signs by decreasing innate and adaptive immune responses in collagen-induced arthritis in mice. We also report that ROS production by circulating classical and non-classical monocytes from patients with RA negatively correlate with disease symptoms. Therefore, ROS produced by different monocyte subsets in peripheral blood might be considered as useful biomarkers or predictors of the immune response associated with RA disease activity. © 2025 European Federation of Immunological Societies. Published by Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.  aINIA LA ESTANZUELA  aMacrophage  aMonocyte  aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA  aReactive oxygen species  aRheumatoid arthritis1 aCOSTA, M.1 aSARAVIA, A.1 aLANDEIRA, M.1 aDA COSTA, V.1 aRODRÍGUEZ-ZRAQUIA, S.A.1 aCEDRÉS, M.E.1 aOLIVA, J.1 aRADO, G.1 aGARCÍA, I.1 aFESTARI, M.F.1 aCONSANI, S.1 aFREIRE, T.  tImmunology Letters, December 2025, Volume 276, 107052. https://doi.org/10.1016/j.imlet.2025.107052