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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
21/02/2014 |
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Actualizado : |
30/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DALLA RIZZA, M.; REAL, D.; QUESENBERRY, K.H.; ALBERTINI, E. |
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Afiliación : |
MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DANIEL REAL FERREIRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; K.H. QUESENBERRY, Dep. of Agronomy, Univ. of Florida, USA; E. ALBERTINI, Dipartimento di Biologia Vegetale e Biotecnologie Agroambientali, University of Perugia, Italy. |
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Título : |
Plant reproductive system determination under field conditions based on condominant markers. |
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Fecha de publicación : |
2004 |
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Fuente / Imprenta : |
Journal of Genetics and Breeding, 2004, Volume 58, Issue 1, pp 47-54. |
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Descripción física : |
2-s2.0-33748791330 |
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ISSN : |
0394-9257 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received February 9, 2004. |
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Contenido : |
ABSTRACT.
Two co-dominant PCR-based molecular tools, sequence characterized amplified regions (SCAR) markers and restriction enzymes mediated markers, sequence characterized amplified regions (CAPS) markers, have been utilized to study the mating system in Lotononis bainesii Baker. Randomly amplified polymorphic DNA (RAPD) phenotypes - bands on a gel - were selected to transform into specific SCAR genotypes - allelic constitution at a set of loci. Moreover, some monomorphic SCAR genotypes, were made polymorphic through the use of restriction enzymes, in order to analyze allelic contribution on progenies. Based on stringent annealing temperatures that generate only specific DNA fragments and only in those individuals possessing the appropriate allele, it was possible to recognize alleles from different donors. This approach overcomes the limitation of different dominant markers and has the advantage of analyzing a single genetic locus PCR-based, avoiding the necessity of complex and time consuming controlled crosses. Thus, more suitable for reproductive system studies based on the use of molecular characterized plants that acts as "tester genotypes". In this article we discuss the advantages of using two independent and conclusive molecular approaches based on SCAR and CAPS markers to determine plant breeding systems. We also show that allogamy is the mode of reproduction of Lotononis bainesii. |
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Palabras claves : |
Mating system; SCAR/CAPS genotypes. |
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Thesagro : |
LOTONONIS BAINESII. |
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Asunto categoría : |
F03 Producción y tratamiento de semillas |
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Marc : |
LEADER 02137naa a2200229 a 4500
001 1012504
005 2019-10-30
008 2004 bl uuuu u00u1 u #d
022 $a0394-9257
100 1 $aDALLA RIZZA, M.
245 $aPlant reproductive system determination under field conditions based on condominant markers.$h[electronic resource]
260 $c2004
300 $c2-s2.0-33748791330
500 $aArticle history: Received February 9, 2004.
520 $aABSTRACT. Two co-dominant PCR-based molecular tools, sequence characterized amplified regions (SCAR) markers and restriction enzymes mediated markers, sequence characterized amplified regions (CAPS) markers, have been utilized to study the mating system in Lotononis bainesii Baker. Randomly amplified polymorphic DNA (RAPD) phenotypes - bands on a gel - were selected to transform into specific SCAR genotypes - allelic constitution at a set of loci. Moreover, some monomorphic SCAR genotypes, were made polymorphic through the use of restriction enzymes, in order to analyze allelic contribution on progenies. Based on stringent annealing temperatures that generate only specific DNA fragments and only in those individuals possessing the appropriate allele, it was possible to recognize alleles from different donors. This approach overcomes the limitation of different dominant markers and has the advantage of analyzing a single genetic locus PCR-based, avoiding the necessity of complex and time consuming controlled crosses. Thus, more suitable for reproductive system studies based on the use of molecular characterized plants that acts as "tester genotypes". In this article we discuss the advantages of using two independent and conclusive molecular approaches based on SCAR and CAPS markers to determine plant breeding systems. We also show that allogamy is the mode of reproduction of Lotononis bainesii.
650 $aLOTONONIS BAINESII
653 $aMating system
653 $aSCAR/CAPS genotypes
700 1 $aREAL, D.
700 1 $aQUESENBERRY, K.H.
700 1 $aALBERTINI, E.
773 $tJournal of Genetics and Breeding, 2004, Volume 58, Issue 1, pp 47-54.
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Registro original : |
INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
25/09/2020 |
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Actualizado : |
27/01/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CARMO, P.M.S.D.; UZAL , F.A.; PEDROSO, P.M.O.; RIET-CORREA, F. |
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Afiliación : |
PRISCILA MARIA SILVA DO CARMO, Hospital Veterinário, Centro de Saúde e Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Bairro Santa Cecília, Patos, Brazil.; FRANCISCO A. UZAL, California Animal Health and Food Safety Laboratory, University of California?Davis, San Bernardino, CA; PEDRO M. O. PEDROSO L, Laboratório de Patologia Veterinária, Faculdade de Veterinária, Universidade Nacional de Brasília, Brasília, Brazil.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Conidiobolomycosis, cryptococcosis, and aspergillosis in sheep and goats: a review. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Journal of Veterinary Diagnostic Investigation, Vol. 32, Issue 6, pag.826-834, Nov. 2020. Doi: https://doi.org/10.1177/1040638720958338 |
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DOI : |
10.1177/1040638720958338 |
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Idioma : |
Inglés |
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Notas : |
Article history: First Published September 14, 2020. |
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Contenido : |
Abstract. We review herein infections by Conidiobolus spp., Cryptococcus spp., and Aspergillus spp. in sheep and goats. Conidiobolus spp. are common causes of rhinitis in sheep and are less frequent in goats, in which Conidiobolus spp. also cause skin lesions. Cryptococcus spp. cause rhinitis, meningitis, encephalitis, and pneumonia in goats, and are rarely observed in sheep. Aspergillus spp. may cause rhinitis in goats, and pneumonia and mastitis in sheep and goats. Gross and microscopic lesions caused by these 3 fungal infections may be similar to each other. The diagnosis of these diseases must be based on gross and microscopic lesions, coupled with detection of the agent by immunohistochemical, molecular, and/or culture-based methods. |
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Palabras claves : |
ASPERGILLUS; CONIDIOBOLUS Spp; CRYPTOCOCCUS SPP; GOATS; PLATAFORMA DE SALUD ANIMAL; SHEEP. |
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Thesagro : |
CABRAS; OVEJAS. |
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Asunto categoría : |
E16 Enfermedades de los animales |
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Marc : |
LEADER 01649naa a2200277 a 4500
001 1061347
005 2021-01-27
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1177/1040638720958338$2DOI
100 1 $aCARMO, P.M.S.D.
245 $aConidiobolomycosis, cryptococcosis, and aspergillosis in sheep and goats$ba review.$h[electronic resource]
260 $c2020
500 $aArticle history: First Published September 14, 2020.
520 $aAbstract. We review herein infections by Conidiobolus spp., Cryptococcus spp., and Aspergillus spp. in sheep and goats. Conidiobolus spp. are common causes of rhinitis in sheep and are less frequent in goats, in which Conidiobolus spp. also cause skin lesions. Cryptococcus spp. cause rhinitis, meningitis, encephalitis, and pneumonia in goats, and are rarely observed in sheep. Aspergillus spp. may cause rhinitis in goats, and pneumonia and mastitis in sheep and goats. Gross and microscopic lesions caused by these 3 fungal infections may be similar to each other. The diagnosis of these diseases must be based on gross and microscopic lesions, coupled with detection of the agent by immunohistochemical, molecular, and/or culture-based methods.
650 $aCABRAS
650 $aOVEJAS
653 $aASPERGILLUS
653 $aCONIDIOBOLUS Spp
653 $aCRYPTOCOCCUS SPP
653 $aGOATS
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aSHEEP
700 1 $aUZAL , F.A.
700 1 $aPEDROSO, P.M.O.
700 1 $aRIET-CORREA, F.
773 $tJournal of Veterinary Diagnostic Investigation, Vol. 32, Issue 6, pag.826-834, Nov. 2020. Doi: https://doi.org/10.1177/1040638720958338
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