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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
02/05/2025 |
Actualizado : |
02/05/2025 |
Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
Autor : |
ARRUABARRENA, A.; VIDAL, S. |
Afiliación : |
ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0002-2781-3613; SABINA VIDAL, Laboratorio de Biología Molecular Vegetal, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay. |
Título : |
Agrobacterium-mediated transformation for gene editing tomato elite breeding lines. |
Complemento del título : |
Protocol. Chapter 12. |
Fecha de publicación : |
2025 |
Fuente / Imprenta : |
In: Stange Klein, C. (eds). Agrobacterium. Methods in Molecular Biology, vol 2911. Humana, New York, NY. pp. 121-131. https://doi.org/10.1007/978-1-0716-4450-8_12 |
Serie : |
Springer Protocols (Methods in Molecular Biology, 2911) |
ISBN : |
978-1-0716-4449-2; e-ISBN: 978-1-0716-4450-8 |
ISSN : |
1064-3745 |
DOI : |
10.1007/978-1-0716-4450-8_12 |
Idioma : |
Inglés |
Notas : |
Chapter history: Published 28 March 2025. -- Correspondence: Arruabarrena, A.; Instituto Nacional de Investigación Agropecuaria, Estación Experimental INIA Salto Grande, Salto, Uruguay; email:aarruabarrena@inia.org.uy -- Funding: The authors thank the INIA Tomato Breeding Program for providing elite genotypes for in vitro regeneration and transformation adjustments, and the INIA (L1 HO_20_0_00) and CSIC (CSIC I+D 1492, UdelaR) projects for funding. -- Publisher: Humana Press Inc. |
Contenido : |
ABSTRACT.- Genome editing success in crop species is largely dependent on the availability of highly efficient plant transformation protocols. Tomato (Solanum lycopersicum) was the first dicotyledonous crop to be successfully mutagenized using CRISPR-Cas9. Despite many efforts, no standardized, simple protocol is available for non-model tomato genotypes. With the increasing availability of gene editing tools, the transformation of elite tomato breeding lines has gained importance because mutant variants can be easily incorporated into breeding programs. This chapter describes a protocol for transforming and gene editing in elite tomato breeding lines, reaching 3.6% transformation efficiency. © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025. |
Palabras claves : |
Agrobacterium tumefaciens; CRISPR-Cas9; Hypocotyl; SISTEMA VEGETAL INTENSIVO - INIA; Solanum lycopersicum. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 02169naa a2200241 a 4500 001 1065176 005 2025-05-02 008 2025 bl uuuu u00u1 u #d 022 $a1064-3745 024 7 $a10.1007/978-1-0716-4450-8_12$2DOI 100 1 $aARRUABARRENA, A. 245 $aAgrobacterium-mediated transformation for gene editing tomato elite breeding lines.$h[electronic resource] 260 $c2025 490 $aSpringer Protocols (Methods in Molecular Biology, 2911) 500 $aChapter history: Published 28 March 2025. -- Correspondence: Arruabarrena, A.; Instituto Nacional de Investigación Agropecuaria, Estación Experimental INIA Salto Grande, Salto, Uruguay; email:aarruabarrena@inia.org.uy -- Funding: The authors thank the INIA Tomato Breeding Program for providing elite genotypes for in vitro regeneration and transformation adjustments, and the INIA (L1 HO_20_0_00) and CSIC (CSIC I+D 1492, UdelaR) projects for funding. -- Publisher: Humana Press Inc. 520 $aABSTRACT.- Genome editing success in crop species is largely dependent on the availability of highly efficient plant transformation protocols. Tomato (Solanum lycopersicum) was the first dicotyledonous crop to be successfully mutagenized using CRISPR-Cas9. Despite many efforts, no standardized, simple protocol is available for non-model tomato genotypes. With the increasing availability of gene editing tools, the transformation of elite tomato breeding lines has gained importance because mutant variants can be easily incorporated into breeding programs. This chapter describes a protocol for transforming and gene editing in elite tomato breeding lines, reaching 3.6% transformation efficiency. © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025. 653 $aAgrobacterium tumefaciens 653 $aCRISPR-Cas9 653 $aHypocotyl 653 $aSISTEMA VEGETAL INTENSIVO - INIA 653 $aSolanum lycopersicum 700 1 $aVIDAL, S. 773 $tIn: Stange Klein, C. (eds). Agrobacterium. Methods in Molecular Biology, vol 2911. Humana, New York, NY. pp. 121-131. https://doi.org/10.1007/978-1-0716-4450-8_12
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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha actual : |
30/05/2019 |
Actualizado : |
04/05/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
WALLACE, F.; BENNADJI, Z.; FERREIRA, F.; OLIVARO, C. |
Afiliación : |
FEDERICO WALLACE, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay.; ZOHRA BENNADJI SOUALHIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO AMARY FERREIRA CHIESA, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay; Laboratorio de Carbohidratos y Glicoconjugados, Departamento de Química Orgánica, Facultad de Química, Udelar, Instituto de Higiene, Uruguay.; CRISTINA OLIVARO, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay. |
Título : |
Structural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry.
. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
Phytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837 |
DOI : |
10.1002/pca.2837 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00);
the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química. |
Contenido : |
Abstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work. MenosAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methyla... Presentar Todo |
Palabras claves : |
IMMUNOADJUVANT SAPONINS; MASS SPECTROMETRY; METHYLATION ANALYSIS; MONOSACCHARIDE ANALYIS; QUILLAJA BRASILIENSIS. |
Asunto categoría : |
K11 Ingeniería forestal |
Marc : |
LEADER 02910naa a2200241 a 4500 001 1059792 005 2020-05-04 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1002/pca.2837$2DOI 100 1 $aWALLACE, F. 245 $aStructural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry. .$h[electronic resource] 260 $c2019 500 $aArticle history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00); the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química. 520 $aAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work. 653 $aIMMUNOADJUVANT SAPONINS 653 $aMASS SPECTROMETRY 653 $aMETHYLATION ANALYSIS 653 $aMONOSACCHARIDE ANALYIS 653 $aQUILLAJA BRASILIENSIS 700 1 $aBENNADJI, Z. 700 1 $aFERREIRA, F. 700 1 $aOLIVARO, C. 773 $tPhytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837
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