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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
02/07/2021 |
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Actualizado : |
02/07/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DE LORENZO, C.; BOABAID, F. M.; OLIVEIRA, L.G.S.; BIANCHI, M. V.; FÉLIX, M.L.; ARMÚA-FERNÁNDEZ, M.T.; SOARES, J.F.; VENZAL, J.M.; SONNE, L. |
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Afiliación : |
CÍNTIA DE LORENZO, Setor de Patologia Veterinária, Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária (FAVET), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil; FABIANA MARQUES BOABAID, Polo de Desarrollo Universitario del Instituto Superior de la Carne, Sede Tacuarembó, Centro Universitario Regional (CENUR) Noreste, Universidad de la República, Tacuarembó, Uruguay; LUIZ GUSTAVO SCHNEIDER DE OLIVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; MATHEUS VIEZZER BIANCHI, Setor de Patologia Veterinária, Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária (FAVET), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil; MARÍA L. FÉLIX, Laboratorio de Vectores y enfermedades transmitidas, Departamento de Ciencias Biológicas, CENUR Litoral Norte – Salto, Universidad de la República, Salto, Uruguay; MARÍA T. ARMÚA-FERNÁNDEZ, Laboratorio de Vectores y enfermedades transmitidas, Departamento de Ciencias Biológicas, CENUR Litoral Norte – Salto, Universidad de la República, Salto, Uruguay; JOAO FABIO SOARES, Laboratório de Protozoologia e Rickettsioses Vetoriais (ProtozooVet), Departamento de Patologia Clínica Veterinária, FAVET, UFRGS, Porto Alegre, Rio Grande do Sul, RS, Brazil; JOSÉ M. VENZAL, Laboratório de Protozoologia e Rickettsioses Vetoriais (ProtozooVet), Departamento de Patologia Clínica Veterinária, FAVET, UFRGS, Porto Alegre, Rio Grande do Sul, RS, Brazil; LUCIANA SONNE, Setor de Patologia Veterinária, Departamento de Patologia Clínica Veterinária, Faculdade de Veterinária (FAVET), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil. |
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Título : |
Rangelia vitalii in free-living crab-eating foxes (Cerdocyon thous) in Uruguay. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Ticks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101765. Doi: https://doi.org/10.1016/j.ttbdis.2021.101765 |
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ISSN : |
1877-959X |
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DOI : |
10.1016/j.ttbdis.2021.101765 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 25 November 2020; Received in revised form 1 May 2021; Accepted 13 May 2021; Available online 4 June 2021.
This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico ( CNPq ) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ( CAPES 001 ).
Corresponding author: Soares, J.F.; Laboratório de Protozoologia e Rickettsioses Vetoriais (ProtozooVet), UFRGS, Avenida Bento Gonçalves, Porto Alegre, Rio Grande do Sul, Brazil; email:joao.soares@ufrgs.br |
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Contenido : |
ABSTRACT - Rangelia vitalii is a protozoan parasite that causes a hemorrhagic and hemolytic disease in dogs known as rangeliosis. Current reports of the disease are concentrated in the southern and southeastern regions of Brazil, as well as in Uruguay, Argentina, and Paraguay, and mainly concern domestic dogs. South American wild canids, such as the crab-eating fox (Cerdocyon thous), the pampas fox (Lycalopex gymnocercus), and the maned wolf (Chrysocyon brachyurus) may also be affected, although existing reports are restricted to Brazil. The present study aimed to detect R. vitalii parasitism in the Uruguayan wild fox population. DNA extracted from the blood and/or spleen samples of road-killed C. thous and L. gymnocercus found in northern Uruguay were subjected to polymerase chain reaction (PCR) to amplify a 551-bp fragment of the Rangelia 18S rRNA gene. A total of 62 wild canids, including 38 C. thous and 24L. gymnocercus, were analyzed. Five crab-eating fox samples (13.2%) were positive for R. vitalii, with 99.5?100% identity between the sequences. All samples from pampas fox tested negative for R. vitalii. When compared with the R. vitalii sequences available in GenBank, a similarity of 98.9?100% was revealed. Molecular analysis results suggest that R. vitalii is circulating in the crab-eating fox population in Uruguay; however, its veterinary relevance for these foxes remains unknown. © 2021 |
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Palabras claves : |
DNA; Dog; Hemoparasites; Rangelia vitalii; Tick-borne disease; Wild canids. |
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Asunto categoría : |
A50 Investigación agraria |
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Marc : |
LEADER 02910naa a2200325 a 4500
001 1062216
005 2021-07-02
008 2021 bl uuuu u00u1 u #d
022 $a1877-959X
024 7 $a10.1016/j.ttbdis.2021.101765$2DOI
100 1 $aDE LORENZO, C.
245 $aRangelia vitalii in free-living crab-eating foxes (Cerdocyon thous) in Uruguay.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 25 November 2020; Received in revised form 1 May 2021; Accepted 13 May 2021; Available online 4 June 2021. This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico ( CNPq ) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ( CAPES 001 ). Corresponding author: Soares, J.F.; Laboratório de Protozoologia e Rickettsioses Vetoriais (ProtozooVet), UFRGS, Avenida Bento Gonçalves, Porto Alegre, Rio Grande do Sul, Brazil; email:joao.soares@ufrgs.br
520 $aABSTRACT - Rangelia vitalii is a protozoan parasite that causes a hemorrhagic and hemolytic disease in dogs known as rangeliosis. Current reports of the disease are concentrated in the southern and southeastern regions of Brazil, as well as in Uruguay, Argentina, and Paraguay, and mainly concern domestic dogs. South American wild canids, such as the crab-eating fox (Cerdocyon thous), the pampas fox (Lycalopex gymnocercus), and the maned wolf (Chrysocyon brachyurus) may also be affected, although existing reports are restricted to Brazil. The present study aimed to detect R. vitalii parasitism in the Uruguayan wild fox population. DNA extracted from the blood and/or spleen samples of road-killed C. thous and L. gymnocercus found in northern Uruguay were subjected to polymerase chain reaction (PCR) to amplify a 551-bp fragment of the Rangelia 18S rRNA gene. A total of 62 wild canids, including 38 C. thous and 24L. gymnocercus, were analyzed. Five crab-eating fox samples (13.2%) were positive for R. vitalii, with 99.5?100% identity between the sequences. All samples from pampas fox tested negative for R. vitalii. When compared with the R. vitalii sequences available in GenBank, a similarity of 98.9?100% was revealed. Molecular analysis results suggest that R. vitalii is circulating in the crab-eating fox population in Uruguay; however, its veterinary relevance for these foxes remains unknown. © 2021
653 $aDNA
653 $aDog
653 $aHemoparasites
653 $aRangelia vitalii
653 $aTick-borne disease
653 $aWild canids
700 1 $aBOABAID, F. M.
700 1 $aOLIVEIRA, L.G.S.
700 1 $aBIANCHI, M. V.
700 1 $aFÉLIX, M.L.
700 1 $aARMÚA-FERNÁNDEZ, M.T.
700 1 $aSOARES, J.F.
700 1 $aVENZAL, J.M.
700 1 $aSONNE, L.
773 $tTicks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101765. Doi: https://doi.org/10.1016/j.ttbdis.2021.101765
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
06/06/2019 |
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Actualizado : |
07/06/2019 |
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Tipo de producción científica : |
Poster |
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Autor : |
RAFFO, M.A.; PRITSCH, C.; AZZIMONTI, G.; PEREYRA, S.; QUINCKE, M.; BONNECARRERE, V.; SILVA, P.; CASTRO, A.; LADO, B.; BARAIBAR, S.; GARCIA, R.; GERMAN, S. |
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Afiliación : |
MIGUEL ANGEL RAFFO BUSCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CLARA PRITSCH; GUSTAVO AZZIMONTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN CONRADO QUINCKE WALDEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA PAULA SILVA VILLELLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ARIEL CASTRO; BETTINA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVINA BARAIBAR PEDERSEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RICHARD ANSELMO GARCIA USUCA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Introgression of the Coupled Sr2-Fhb1for Resistance to Stem Rust and Fusarium Head Blight into Uruguayan Elite Wheat Cultivars.[Poster] |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
In: Proceedings of the Borlaug Global Rust Initiative Technical Workshop ,14-18 April, 2018, Marrakesh, Marruecos. |
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Idioma : |
Inglés |
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Contenido : |
Abstract: Stem rust (SR) and Fusarium head blight (FHB) threaten the sustainability of wheat production worldwide. Sr2 is a widely used gene conferring partial, but durable, resistance to SR. Fhb1 confers a significant level of FHB resistance, but is poorly represented in the INIA-Uruguay wheat-breeding program. Sr2 and Fhb1 are linked in repulsion (~3 cM apart) on chromosome 3B. However, lines with Sr2 and Fhb1 in coupling were recently developed at the University of Minnesota, USA (kindly provided by J. Anderson). In order to incorporate Sr2/Fhb1 into Uruguayan elite wheat cultivars the donor line was crossed and backcrossed with four cultivars lacking both genes and expressing an intermediate to low level of resistance to SR and FHB: G?nesis 2375, G?nesis 6.87, INIA Madrugador, and INIA Don Alberto. Genotypes carrying Sr2/Fhb1 were selected using molecular marker UMN10; 250 BC2F1 were obtained for each recurrent parent. BC3F1 plants positive for UMN10 will be selected. The effect of Sr2/Fhb1 on response to SR and FHB in the different genetic backgrounds will be quantified by comparing disease severities of BC3F2 homozygotes with and without the UMN10 marker. Hopefully the introduction of Sr2/Fhb1 will contribute in reducing the risk of SR and FHB in wheat crops in Uruguay. |
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Palabras claves : |
FUSARIUM; PLATAFORMA FENOTIPADO DE TRIGO; RESISTENCIA ROYA DEL TALLO; STEM RUST; TIZÓN FOLIAR. |
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Thesagro : |
TRIGO. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02276nam a2200313 a 4500
001 1059828
005 2019-06-07
008 2018 bl uuuu u00u1 u #d
100 1 $aRAFFO, M.A.
245 $aIntrogression of the Coupled Sr2-Fhb1for Resistance to Stem Rust and Fusarium Head Blight into Uruguayan Elite Wheat Cultivars.[Poster]$h[electronic resource]
260 $aIn: Proceedings of the Borlaug Global Rust Initiative Technical Workshop ,14-18 April, 2018, Marrakesh, Marruecos.$c2018
520 $aAbstract: Stem rust (SR) and Fusarium head blight (FHB) threaten the sustainability of wheat production worldwide. Sr2 is a widely used gene conferring partial, but durable, resistance to SR. Fhb1 confers a significant level of FHB resistance, but is poorly represented in the INIA-Uruguay wheat-breeding program. Sr2 and Fhb1 are linked in repulsion (~3 cM apart) on chromosome 3B. However, lines with Sr2 and Fhb1 in coupling were recently developed at the University of Minnesota, USA (kindly provided by J. Anderson). In order to incorporate Sr2/Fhb1 into Uruguayan elite wheat cultivars the donor line was crossed and backcrossed with four cultivars lacking both genes and expressing an intermediate to low level of resistance to SR and FHB: G?nesis 2375, G?nesis 6.87, INIA Madrugador, and INIA Don Alberto. Genotypes carrying Sr2/Fhb1 were selected using molecular marker UMN10; 250 BC2F1 were obtained for each recurrent parent. BC3F1 plants positive for UMN10 will be selected. The effect of Sr2/Fhb1 on response to SR and FHB in the different genetic backgrounds will be quantified by comparing disease severities of BC3F2 homozygotes with and without the UMN10 marker. Hopefully the introduction of Sr2/Fhb1 will contribute in reducing the risk of SR and FHB in wheat crops in Uruguay.
650 $aTRIGO
653 $aFUSARIUM
653 $aPLATAFORMA FENOTIPADO DE TRIGO
653 $aRESISTENCIA ROYA DEL TALLO
653 $aSTEM RUST
653 $aTIZÓN FOLIAR
700 1 $aPRITSCH, C.
700 1 $aAZZIMONTI, G.
700 1 $aPEREYRA, S.
700 1 $aQUINCKE, M.
700 1 $aBONNECARRERE, V.
700 1 $aSILVA, P.
700 1 $aCASTRO, A.
700 1 $aLADO, B.
700 1 $aBARAIBAR, S.
700 1 $aGARCIA, R.
700 1 $aGERMAN, S.
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