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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
28/04/2025 |
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Actualizado : |
28/04/2025 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MORALES, D. F.; PARODI, P.; DOS REIS, L. F. V.; NASCIMENTO, M. C.; RODRIGUES, G. D.; NUNES, L. S.; MENCHACA, A.; RIET-CORREA, F.; KROLOW, T. K.; LUCAS, M.; SARAVIA, A.; DOMINGUES, W. B.; CAMPOS, V. F.; KRÜGER, R. F. |
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Afiliación : |
DIULIANI FONSECA MORALES, Ecology of Parasites and Vectors Group, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil; PABLO ANDRÉS PARODI TEXEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUANA FERREIRA VIANA DOS REIS, Structural Genomics Group, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil; MARIANA CAVALCANTI NASCIMENTO, Structural Genomics Group, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil; GRATCHELA DUTRA RODRIGUES, Ecology of Parasites and Vectors Group, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil; LEANDRO SILVA NUNES, Structural Genomics Group, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0002-2494-9574; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0001-5738-7785; TIAGO KÜTTER KROLOW, Programa de Pós?Graduação em Biodiversidade, Ecologia e Conservação (PPGBEC), Universidade Federal do Tocantins, Tocantins, Brazil; MARTÍN LUCAS FONSECA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; ANDERSON SARAVIA DE MELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0002-3624-2437; WILLIAM BORGES DOMINGUES, Structural Genomics Group, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil; VINICIUS FARIAS CAMPOS, Structural Genomics Group, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil; RODRIGO FERREIRA KRÜGER, Ecology of Parasites and Vectors Group, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil. |
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Título : |
First molecular detection of Babesia bigemina in horseflies from Uruguay. |
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Fecha de publicación : |
2025 |
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Fuente / Imprenta : |
Acta Tropica, April 2025, Volume 264, 107595. https://doi.org/10.1016/j.actatropica.2025.107595 |
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ISSN : |
0001-706X |
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DOI : |
10.1016/j.actatropica.2025.107595 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 19 November 2024, Revised 17 February 2025, Accepted 21 March 2025, Available online 24 March 2025, Version of Record 29 March 2025. -- Corresponding author: E-mail address: diulimoralesfonseca@gmail.com (D.F. Morales). -- |
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Contenido : |
ABSTRACT.- Bovine babesiosis and anaplasmosis are primarily transmitted by the biological vector Rhipicephalus microplus. However, Anaplasma marginale may also be mechanically transmitted through the bites of haematophagous flies such as tabanids. While biological transmission by ticks is the predominant route for Babesia species, some reports suggest that mechanical transmission via fomites contaminated with merozoites may occur. Additionally, mechanical vectors such as tabanids may contribute to the spread of the disease. This study investigated the presence of Babesia bigemina and Babesia bovis in tabanids from three localities in Uruguay previously evaluated
for the presence of A. marginale. We performed PCR amplification targeting the Rap-1a and gp45 genes, followed by Sanger sequencing. Our findings revealed that 4.21 % of the tabanids tested positive for B. bigemina, with the highest prevalence in Tacuarembó (28.57 %), followed by Paysandú (3.22 %) and Colonia (1.75 %). Dasybasis
missionum from Tacuarembó, Poeciloderas lindneri from Paysandú, and Tabanus sp. from Colonia were positive. Additionally, a co-detection rate of 2 % with A. marginale was observed. These findings underscore the potential role of tabanids as vectors for Babesia bigemina, emphasizing the need for integrated vector control strategies in
livestock management. © 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies. |
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Palabras claves : |
Anaplasmosis; Babesiosis; Mechanical transmission, tabanids; Pathogen co-detection; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 02862naa a2200373 a 4500
001 1065170
005 2025-04-28
008 2025 bl uuuu u00u1 u #d
022 $a0001-706X
024 7 $a10.1016/j.actatropica.2025.107595$2DOI
100 1 $aMORALES, D. F.
245 $aFirst molecular detection of Babesia bigemina in horseflies from Uruguay.$h[electronic resource]
260 $c2025
500 $aArticle history: Received 19 November 2024, Revised 17 February 2025, Accepted 21 March 2025, Available online 24 March 2025, Version of Record 29 March 2025. -- Corresponding author: E-mail address: diulimoralesfonseca@gmail.com (D.F. Morales). --
520 $aABSTRACT.- Bovine babesiosis and anaplasmosis are primarily transmitted by the biological vector Rhipicephalus microplus. However, Anaplasma marginale may also be mechanically transmitted through the bites of haematophagous flies such as tabanids. While biological transmission by ticks is the predominant route for Babesia species, some reports suggest that mechanical transmission via fomites contaminated with merozoites may occur. Additionally, mechanical vectors such as tabanids may contribute to the spread of the disease. This study investigated the presence of Babesia bigemina and Babesia bovis in tabanids from three localities in Uruguay previously evaluated for the presence of A. marginale. We performed PCR amplification targeting the Rap-1a and gp45 genes, followed by Sanger sequencing. Our findings revealed that 4.21 % of the tabanids tested positive for B. bigemina, with the highest prevalence in Tacuarembó (28.57 %), followed by Paysandú (3.22 %) and Colonia (1.75 %). Dasybasis missionum from Tacuarembó, Poeciloderas lindneri from Paysandú, and Tabanus sp. from Colonia were positive. Additionally, a co-detection rate of 2 % with A. marginale was observed. These findings underscore the potential role of tabanids as vectors for Babesia bigemina, emphasizing the need for integrated vector control strategies in livestock management. © 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
653 $aAnaplasmosis
653 $aBabesiosis
653 $aMechanical transmission, tabanids
653 $aPathogen co-detection
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
700 1 $aPARODI, P.
700 1 $aDOS REIS, L. F. V.
700 1 $aNASCIMENTO, M. C.
700 1 $aRODRIGUES, G. D.
700 1 $aNUNES, L. S.
700 1 $aMENCHACA, A.
700 1 $aRIET-CORREA, F.
700 1 $aKROLOW, T. K.
700 1 $aLUCAS, M.
700 1 $aSARAVIA, A.
700 1 $aDOMINGUES, W. B.
700 1 $aCAMPOS, V. F.
700 1 $aKRÜGER, R. F.
773 $tActa Tropica, April 2025, Volume 264, 107595. https://doi.org/10.1016/j.actatropica.2025.107595
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
21/07/2025 |
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Actualizado : |
21/07/2025 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
VILARÓ, M.; REYNO, R.; CONDON, F.; DO CANTO, J.; TRUJILLO, C.; BARRIOS, P.G.; RIVAS, M. |
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Afiliación : |
MARIANA VILARÓ, Centro Universitario Regional del Este, Ruta Nacional No. 9 intersección con Ruta No. 15, Rocha, Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0001-9619-8477; FEDERICO CONDON PRIANO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0002-3374-3607; JAVIER DO CANTO FAGUNDEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORCID: https://orcid.org/0000-0003-2268-5592; CRISTINA TRUJILLO, Facultad de Agronomía, Universidad de La República, Avda. Garzón 780, Montevideo, Uruguay; PABLO GONZÁLEZ BARRIOS, Facultad de Agronomía, Universidad de La República, Avda. Garzón 780, Montevideo, Uruguay; MERCEDES RIVAS, Centro Universitario Regional del Este, Ruta Nacional No. 9 intersección con Ruta No. 15, Rocha, Uruguay; Facultad de Agronomía, Universidad de La República, Avda. Garzón 780, Montevideo, Uruguay. |
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Título : |
New insights into the breeding system of Bromus auleticus (Poaceae): pollination methods and reproductive success. (Research article). |
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Fecha de publicación : |
2025 |
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Fuente / Imprenta : |
Genetic Resources and Crop Evolution, 2025. https://doi.org/10.1007/s10722-025-02505-w -- [Article In Press]. |
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ISSN : |
0925-9864 |
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DOI : |
https://doi.org/10.1007/s10722-025-02505-w |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 29 April 2025; Accepted 26 May 2025; Published online 18 June 2025. -- Corresponding author: M. Vilaró, Centro Universitario Regional del Este, Ruta Nacional No. 9 intersección con Ruta No. 15, Rocha, Uruguay, Email: mvilaro@fagro.edu.uy -- Funding: This work was partially funded by the Comisión
Sectorial de Investigación Científica - CSIC (Sectoral Committee for Scientific Research) of the Universidad de la República (University of the Republic), Centro Universitario Regional del Este - CURE Rocha (Eastern Regional University Centre) and Instituto Nacional de Investigación Agropecuaria - INIA Uruguay (National Institute for Agricultural Research). The first author received financial support from the Graduate Academic Committee (Comisión Académica de Posgrados) of the University of the Republic. -- Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1007/s10722- 025- 02505-w . -- |
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Contenido : |
ABSTRACT.- Bromus auleticus is a perennial grass native to the campos of South America, valued for its persistence, productivity and forage quality. Conservation and breeding efforts are constrained by limited knowledge of its reproductive aspects. Although allogamy and genetic incompatibility have been reported, these traits have not been sufficiently corroborated across multiple populations. This work aimed to determine the reproductive system of B. auleticus, assess the presence of genetic incompatibility and explore the possibility of obtaining recombinants between contrasting ecotypes. Between 2018 and 2022, seed set and germination of seeds obtained with different pollination methods were compared, pollen-pistil interactions and pollen viability analysis were performed and a hybridization technique was developed. Open pollination treatments showed the highest seed set (48.7%) and germination (56.8%) as well as the highest pollen-pistil compatibility. In self-pollination, the average seed set was 3.13%, germination was intermediate (35.8%) and pollen-pistil interactions were mostly partially compatible or not compatible. These results confirmed that the five accessions show genetic incompatibility, although this mechanism is not strict and requires further study. Inter-accession crosses yielded 1.96% seed set, while intra-accession crosses produced 0.46%. Pollen viability was not a limiting factor in seed formation. This study presents the first report of controlled hybridizations among B. auleticus ecotypes from Uruguay and provides a comprehensive description of the techniques employed. These results have implications for conservation strategies, as well as applications in breeding and seed production, particularly for the selection of crosses with high pollen-stigma compatibility and seed set. © The Author(s), under exclusive licence to Springer Nature B.V. 2025. MenosABSTRACT.- Bromus auleticus is a perennial grass native to the campos of South America, valued for its persistence, productivity and forage quality. Conservation and breeding efforts are constrained by limited knowledge of its reproductive aspects. Although allogamy and genetic incompatibility have been reported, these traits have not been sufficiently corroborated across multiple populations. This work aimed to determine the reproductive system of B. auleticus, assess the presence of genetic incompatibility and explore the possibility of obtaining recombinants between contrasting ecotypes. Between 2018 and 2022, seed set and germination of seeds obtained with different pollination methods were compared, pollen-pistil interactions and pollen viability analysis were performed and a hybridization technique was developed. Open pollination treatments showed the highest seed set (48.7%) and germination (56.8%) as well as the highest pollen-pistil compatibility. In self-pollination, the average seed set was 3.13%, germination was intermediate (35.8%) and pollen-pistil interactions were mostly partially compatible or not compatible. These results confirmed that the five accessions show genetic incompatibility, although this mechanism is not strict and requires further study. Inter-accession crosses yielded 1.96% seed set, while intra-accession crosses produced 0.46%. Pollen viability was not a limiting factor in seed formation. This study presents the first report of controlled hyb... Presentar Todo |
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Palabras claves : |
ÁREA DE PASTURAS Y FORRAJES - INIA; Campos; Forage genetic resources; Genetic incompatibility; Hybridization techniques; INIA LA ESTANZUELA; INIA TACUAREMBÓ; Pollen viability; Seed set; SISTEMA AGRÍCOLA-GANADERO - INIA. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 04024naa a2200349 a 4500
001 1065301
005 2025-07-21
008 2025 bl uuuu u00u1 u #d
022 $a0925-9864
024 7 $ahttps://doi.org/10.1007/s10722-025-02505-w$2DOI
100 1 $aVILARÓ, M.
245 $aNew insights into the breeding system of Bromus auleticus (Poaceae)$bpollination methods and reproductive success. (Research article).$h[electronic resource]
260 $c2025
500 $aArticle history: Received 29 April 2025; Accepted 26 May 2025; Published online 18 June 2025. -- Corresponding author: M. Vilaró, Centro Universitario Regional del Este, Ruta Nacional No. 9 intersección con Ruta No. 15, Rocha, Uruguay, Email: mvilaro@fagro.edu.uy -- Funding: This work was partially funded by the Comisión Sectorial de Investigación Científica - CSIC (Sectoral Committee for Scientific Research) of the Universidad de la República (University of the Republic), Centro Universitario Regional del Este - CURE Rocha (Eastern Regional University Centre) and Instituto Nacional de Investigación Agropecuaria - INIA Uruguay (National Institute for Agricultural Research). The first author received financial support from the Graduate Academic Committee (Comisión Académica de Posgrados) of the University of the Republic. -- Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1007/s10722- 025- 02505-w . --
520 $aABSTRACT.- Bromus auleticus is a perennial grass native to the campos of South America, valued for its persistence, productivity and forage quality. Conservation and breeding efforts are constrained by limited knowledge of its reproductive aspects. Although allogamy and genetic incompatibility have been reported, these traits have not been sufficiently corroborated across multiple populations. This work aimed to determine the reproductive system of B. auleticus, assess the presence of genetic incompatibility and explore the possibility of obtaining recombinants between contrasting ecotypes. Between 2018 and 2022, seed set and germination of seeds obtained with different pollination methods were compared, pollen-pistil interactions and pollen viability analysis were performed and a hybridization technique was developed. Open pollination treatments showed the highest seed set (48.7%) and germination (56.8%) as well as the highest pollen-pistil compatibility. In self-pollination, the average seed set was 3.13%, germination was intermediate (35.8%) and pollen-pistil interactions were mostly partially compatible or not compatible. These results confirmed that the five accessions show genetic incompatibility, although this mechanism is not strict and requires further study. Inter-accession crosses yielded 1.96% seed set, while intra-accession crosses produced 0.46%. Pollen viability was not a limiting factor in seed formation. This study presents the first report of controlled hybridizations among B. auleticus ecotypes from Uruguay and provides a comprehensive description of the techniques employed. These results have implications for conservation strategies, as well as applications in breeding and seed production, particularly for the selection of crosses with high pollen-stigma compatibility and seed set. © The Author(s), under exclusive licence to Springer Nature B.V. 2025.
653 $aÁREA DE PASTURAS Y FORRAJES - INIA
653 $aCampos
653 $aForage genetic resources
653 $aGenetic incompatibility
653 $aHybridization techniques
653 $aINIA LA ESTANZUELA
653 $aINIA TACUAREMBÓ
653 $aPollen viability
653 $aSeed set
653 $aSISTEMA AGRÍCOLA-GANADERO - INIA
700 1 $aREYNO, R.
700 1 $aCONDON, F.
700 1 $aDO CANTO, J.
700 1 $aTRUJILLO, C.
700 1 $aBARRIOS, P.G.
700 1 $aRIVAS, M.
773 $tGenetic Resources and Crop Evolution, 2025. https://doi.org/10.1007/s10722-025-02505-w -- [Article In Press].
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