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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
30/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 5 |
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Autor : |
DALLA RIZZA, M.; REAL, D.; QUESENBERRY, K.H.; ALBERTINI, E. |
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Afiliación : |
MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DANIEL REAL FERREIRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; K.H. QUESENBERRY, Dep. of Agronomy, Univ. of Florida, USA; E. ALBERTINI, Dipartimento di Biologia Vegetale e Biotecnologie Agroambientali, University of Perugia, Italy. |
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Título : |
Plant reproductive system determination under field conditions based on condominant markers. |
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Fecha de publicación : |
2004 |
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Fuente / Imprenta : |
Journal of Genetics and Breeding, 2004, Volume 58, Issue 1, pp 47-54. |
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Descripción física : |
2-s2.0-33748791330 |
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ISSN : |
0394-9257 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received February 9, 2004. |
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Contenido : |
ABSTRACT.
Two co-dominant PCR-based molecular tools, sequence characterized amplified regions (SCAR) markers and restriction enzymes mediated markers, sequence characterized amplified regions (CAPS) markers, have been utilized to study the mating system in Lotononis bainesii Baker. Randomly amplified polymorphic DNA (RAPD) phenotypes - bands on a gel - were selected to transform into specific SCAR genotypes - allelic constitution at a set of loci. Moreover, some monomorphic SCAR genotypes, were made polymorphic through the use of restriction enzymes, in order to analyze allelic contribution on progenies. Based on stringent annealing temperatures that generate only specific DNA fragments and only in those individuals possessing the appropriate allele, it was possible to recognize alleles from different donors. This approach overcomes the limitation of different dominant markers and has the advantage of analyzing a single genetic locus PCR-based, avoiding the necessity of complex and time consuming controlled crosses. Thus, more suitable for reproductive system studies based on the use of molecular characterized plants that acts as "tester genotypes". In this article we discuss the advantages of using two independent and conclusive molecular approaches based on SCAR and CAPS markers to determine plant breeding systems. We also show that allogamy is the mode of reproduction of Lotononis bainesii. |
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Palabras claves : |
Mating system; SCAR/CAPS genotypes. |
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Thesagro : |
LOTONONIS BAINESII. |
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Asunto categoría : |
F03 Producción y tratamiento de semillas |
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Marc : |
LEADER 02137naa a2200229 a 4500
001 1012504
005 2019-10-30
008 2004 bl uuuu u00u1 u #d
022 $a0394-9257
100 1 $aDALLA RIZZA, M.
245 $aPlant reproductive system determination under field conditions based on condominant markers.$h[electronic resource]
260 $c2004
300 $c2-s2.0-33748791330
500 $aArticle history: Received February 9, 2004.
520 $aABSTRACT. Two co-dominant PCR-based molecular tools, sequence characterized amplified regions (SCAR) markers and restriction enzymes mediated markers, sequence characterized amplified regions (CAPS) markers, have been utilized to study the mating system in Lotononis bainesii Baker. Randomly amplified polymorphic DNA (RAPD) phenotypes - bands on a gel - were selected to transform into specific SCAR genotypes - allelic constitution at a set of loci. Moreover, some monomorphic SCAR genotypes, were made polymorphic through the use of restriction enzymes, in order to analyze allelic contribution on progenies. Based on stringent annealing temperatures that generate only specific DNA fragments and only in those individuals possessing the appropriate allele, it was possible to recognize alleles from different donors. This approach overcomes the limitation of different dominant markers and has the advantage of analyzing a single genetic locus PCR-based, avoiding the necessity of complex and time consuming controlled crosses. Thus, more suitable for reproductive system studies based on the use of molecular characterized plants that acts as "tester genotypes". In this article we discuss the advantages of using two independent and conclusive molecular approaches based on SCAR and CAPS markers to determine plant breeding systems. We also show that allogamy is the mode of reproduction of Lotononis bainesii.
650 $aLOTONONIS BAINESII
653 $aMating system
653 $aSCAR/CAPS genotypes
700 1 $aREAL, D.
700 1 $aQUESENBERRY, K.H.
700 1 $aALBERTINI, E.
773 $tJournal of Genetics and Breeding, 2004, Volume 58, Issue 1, pp 47-54.
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INIA Las Brujas (LB) |
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